0000005281 00000 n • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer® Select modifications for enhanced specificity • For use in human, mouse, and rat cells 0000012917 00000 n In brief, 100µL/well of 1× siRNA resuspension buffer was added to the wells of the . 0000425713 00000 n POA For short-term storage, RNase-free water (Cat # B-003000-WB-100) is also appropriate for resuspension of concentrated stocks. 0000004483 00000 n One or more aliquots of 0.1 - 5 nmol siRNA reagent per well Eight or more single-use RTF plates Custom libraries for unique experimental requirements Resuspension recommendations 1. %%EOF 0000005074 00000 n GE Dharmacon RNAi products encompass the most complete portfolio of innovative tools for transient, long-term, inducible and in vivo RNAi applications. siRNA buffer로녹여 – 20°C~–80°C에서 보관할 경우 6개월 동안 안정합니다. 0000003044 00000 n by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions. 0000013559 00000 n The RNA oligo was dried down from a buffered solution. Broadly useful across many applications and in any cell line, siRNA offers a straightforward and widely cited technique for functional gene silencing. 0000001717 00000 n Briefly centrifuge the tube or plate to ensure that the dried siRNA is at the bottom of the tube. 945 plate to give a siRNA concentration of 10 µM. 269 46 Store at -20°C. Available as SMARTpool or Set of 4 2. 6. siRNA resuspension protocol Note: This protocol is written for siRNA, but may also be applied to microRNA mimic and hairpin inhibitor resuspension. The Dharmacon RTF siRNA libraries contain preselected groups of rationally designed SMARTpool siRNA reagents targeting genes confirmed to be relevant to a SiRNA buffer is recommended for long term storage, you can resuspend your SiRNA in RNAse free molecular grade water (or 0.22µm filtered DEPC treated water) for immediate use. 0000033063 00000 n Freeley et al. 0000006570 00000 n 0000432148 00000 n siRNA is dried down in the presence of buffer (show below). 2.2 siRNA resuspension. Briefly centrifuge tubes containing siRNA to ensure that the siRNA pellet is collected at the bottom of the tube. 0000017369 00000 n 이 제품은 별도로 구매를 하셔야 합니다. Dharmacon™ Accell™siRNA delivery 6. This will help with stability of the RNA during freeze-thaw cycles. Resuspension of siRNA duplexes Before removing the thermoseal of the plates, centrifuge each plate at low speed to pellet the dried siRNA at the bottom of each well. Resuspend in RNase-free 1x siRNA Buffer (See note below) for the ON-TARGET plus siRNA pool is used because it contains a pool of four individual siRNA sequences for the target gene (Note 1). GE Healthcare Dharmacon™ ON-TARGETplus™ Non-Targeting siRNAs Ideal negative control for RNAi experiments in human, mouse, and rat cells. Dharmacon Accell Non-targeting Pool() Global Life Sciences Solutions USA LLC/Cytiva VWR International, LLC Fisher Scientific Company LLC Horizon Discovery Group plc US View all 1. siRNA – Pre-designed genome-wide reagents for siGENOME, specificity-enahanced ON- TARGETplus, 2. and self-delivering Accell product lines. Notes: siRNA (and microRNA mimic) should be resuspended in RNase-free solutions. Dharmacon siRNA는 어디에다 녹여야 하나요? 38 0 obj <> endobj • siRNA (and microRNA mimic) should be resuspended in RNase-free solutions. Silencer Select siRNA modifications reduce off-target effects and yield more reliable phenotypic data. h��Y oܸ�� From research to therapy, Horizon Discovery drives the application of gene editing and gene modulation. 5X siRNA Buffer (100 mL) from Horizon Discovery. 0000012528 00000 n 0000425013 00000 n 0000001895 00000 n 0000003847 00000 n 0000011389 00000 n 0000009722 00000 n ZERO BIAS - scores, article reviews, protocol conditions and more For short-term storage, RNase-free water (Cat #B-002000-WB-100) is also appropriate for resuspension of concentrated stocks. a. Resuspend siRNA using the appropriate volume of 1x siRNA Buffer h�b``�f``Kd`e`��cf@ a���S�[� ����v�@��~�aw_5��MCC#::`j::��������v � ��`i�%� �}���!��N\C��|88�x�$&0leP+`��0��� P��A0�qGcS��%��S+��Hp0LfM`J`0b������������>n���@���mق�`������ "�3� endstream endobj 313 0 obj <>/Filter/FlateDecode/Index[20 249]/Length 31/Size 269/Type/XRef/W[1 1 1]>>stream 0000425940 00000 n Briefly centrifuge plates to ensure that the siRNA is collected at the bottom of the well. 0000425382 00000 n Wipe adhesive foil cover with 70% ethanol or other RNase-decontamination solution such as Fisherbrand™ RNase Displace™ Decontaminant (Cat #04 … Leave at room temperature for 5 So your small interfering RNA, or #siRNA, arrived dried and needs to be resuspended before use, so how do you do it? 53 different siRNAs, including older designs previously noted to elicit off-target phenotypes, were transfected into U2OS cells at 30 nM in both unmodified and Silencer Select modified formats. Annealing of siRNA Ambion provides 5X Annealing Buffer with each siRNA. 0000005167 00000 n 0000018277 00000 n 0000014225 00000 n 0000005393 00000 n 0000001536 00000 n 0 Ambion® Silencer® SelectGAPDH Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. Choose highly potent siRNAs for human, mouse, and rat 3. shRNA­ – SMARTvector, GIPZ, TRIPZ and TRC lentiviral shRNA products designed for optimal gene silen… Sirna Resuspension Buffer, supplied by GE Healthcare, used in various techniques. Using its core expertise in chemistry, biology, bioinformatics and production, Dharmacon has pioneered a custom 0000024488 00000 n Specification Name Specification Value; Mfr. 0000423014 00000 n Transfectants To determine the best transfectant for Raw 264.7 cells, 10 different lipofectants were tested. siRNA resuspension buffer (Dharmacon) under sterile con-ditions according to the manufacturer’s recommendations with minor modifications. For a detailed resuspension protocol and tips on accurate spectrophotometry readings see the siRNA Resuspension Protocol. 0000015088 00000 n endstream endobj startxref Ten daughter 2. rapid and economical screening. 0000001953 00000 n 0000007813 00000 n 0000001216 00000 n trailer <<8E4C93FFC8554CEB8A48625EA3E7E3D7>]/Prev 462646/XRefStm 1536>> startxref 0 %%EOF 314 0 obj <>stream 2. siRNA Resuspension Protocol We recommend preparing 100 μM siRNA stock solution. h�b``�a``���������ǀ |��@q���w@BJ�n\g`��`�� /#�{ -Ģ`�^n�{-�M)�*:R��g�b�z���"�3���C�v0��Ҍ@7܆�,��(�e�` ��� Dharmacon Inc., now known as Dharmacon, a Horizon Discovery Group company, was founded in 1995 by Stephen Scaringe as Dharmacon Research to develop and commercialize a new technology for RNA oligonucleotide synthesis.The original Dharmacon focus and vision was to develop 2'-ACE RNA technology as the standard for RNA synthesis and to advance RNA oligo-dependent applications and … Dharmacon BioResearch posted a video to playlist siRNA. 60 0 obj <>/Filter/FlateDecode/ID[<5A1AFA6D4C81C1408C67903E2B2F73D4><5D9CCE87FB4DE04F8D10E71F59F670AA>]/Index[38 54]/Info 37 0 R/Length 104/Prev 73009/Root 39 0 R/Size 92/Type/XRef/W[1 2 1]>>stream h�bbd``b`z$�A�3@����$Xm@�.�`ˁ�&K H��̝ �)@B�H��o@b^ S���R !! We recommend 1x siRNA Buffer (diluted from Dharmacon 5x siRNA Buffer, Cat #B-002000-UB-100). Resuspension to 20 µM will reconstitute the buffer to 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM EDTA. 0000000016 00000 n Product Specs; Item 5X siRNA Buffer (100 mL) Company Horizon Discovery; Catalog Number B-002000-UB-100; This product is no longer available on Biocompare. Dilute the stock 4390843 solution to 10 μM for immediate use. H�\�͎�@��w+3ӽHȒ�cɇ�(�. 0000424692 00000 n 0000009303 00000 n 0000003225 00000 n h�bb�c`b``Ń3� ����&0 Ы5 endstream endobj 270 0 obj <>/Metadata 18 0 R/OpenAction 271 0 R/Pages 17 0 R/StructTreeRoot 20 0 R/Type/Catalog/ViewerPreferences<>>> endobj 271 0 obj <> endobj 272 0 obj >/PageWidthList<0 595.276>>>>>>/Resources<>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/Rotate 0/StructParents 0/TrimBox[0.0 0.0 595.276 790.866]/Type/Page>> endobj 273 0 obj [274 0 R] endobj 274 0 obj <>/Border[0 0 0]/H/N/Rect[385.066 347.482 400.383 337.866]/StructParent 1/Subtype/Link/Type/Annot>> endobj 275 0 obj <> endobj 276 0 obj <>stream

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